Investigative Skills in Science
Research Proposal Form
Project Title: Investigation on how different amount of salt in water affects the hatch rate of brine shrimps.
Class:
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S2-08
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Group:
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K
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SN
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Name of student
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Email
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1
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Chu Tze Hern
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chu_tze_hern@s2018.ssts.edu.sg
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Category of research:
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Sub-category:
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Test a hypothesis: Hypothesis-driven research
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Investigation of the optimal amount of salt in water for the highest number of brine shrimp hatched.
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Reference
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Research Plan
Project Title: How different amounts of salt in water
affects the number of brine shrimps hatched
1. RATIONALE: Include a brief synopsis of the background that supports your research problem and explain why this research is important and if applicable, explain any societal impact of your research.
There is little to no societal impact from this experiment as Brine shrimps or artemia are invertebrates but are part of our animal society thus there is a bit societal impact that then happens from this experiment.
(When the cysts become wet, they begin to swell with water. After about 18 to 24 hours at 78 degrees Fahrenheit, they begin to hatch. Larval shrimp look nothing like adults and are called nauplii. They have a single eye, no mouth, and no anus; so they do not eat Many people know that brine shrimps are essential to the success of their aquarium but do not know how to hatch the brine shrimps. Thus it is necessary that they know the correct amount of salt to add in the water for the highest hatch rate.
This research is vital as if people add too much or too little salt in the water the brine shrimps are hatched in, the brine shrimps will die inside their eggs leading in the loss of lives of animals.
and cannot process food. But they are packed with energy: proteins, highly unsaturated fatty acids, enzymes and more. All of these just happen to be what larval fish need to help them off to a good start.
During the first 12 or so hours of life (called Instar 1), nauplii use this stored energy. They are attracted to light, so they begin swimming near the surface of the water and preparing for their first molt. Immediately after the first molt, the nauplii take in water and swell up before the chitin in their shells hardens. This is the way all crustaceans grow. At this point, they are known as Instar 2, and they have a mouth and anus, so they can begin eating. Their diet is the same as that of the adults, consisting of microscopic free-floating algae, bacteria, and even some detritus, though algae make up the vast majority of their diet. Over the next several days, they molt up to 10 more times before finally reaching adult size and form.) This tells me most of the steps I need to do for the experiment.
2. RESEARCH QUESTION(S):
2.1 Research question being addressed
To find out what the salt concentration in the water is optimal for brine shrimp hatched.
2.2 Hypotheses
The hypothesis is the higher the salt concentration, the higher the amount of shrimp eggs hatched.
2.2.1 Independent variable
The independent variable is the amount of salt added into the water
2.2.2 Dependent variable
The number of empty eggs floating on the surface.
2.2.3 Controlled variables
(a) The amount of water.
(b) The number of eggs.
2.3 How is this based on the rationale described above?
The amount of salt added in the water will affect the number of eggs hatched and this will be used to find out the optimal number of salt that needs to be added into the water.
3. Method
3.1 Equipment list:
- Salt
- Six conical flasks
- Air pump
- Dissecting Microscope
- Teaspoon
3.2 Diagrams
3.3 Procedures:
- Add 250 ml of water
- Connect the air pump to the tubing and put the tubing in the 3 conical flasks and add the respective amounts of salt.
- Turn on the air pump and leave the eggs to settle4. Plot the graph of number of brine shrimps hatched to the number of days
Amount of salt.
- 1/6 teaspoon
- 2/6 teaspoon
- 1/2 teaspoon
- 4/6 teaspoon
- 5/6 teaspoon
- 1 teaspoon
4. Risk assessment
Risk
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Assessment
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Management
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The student may accidentally get the salt water into his eyes
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Medium
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Wear protective eyepieces.
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The air pump may malfunction and cause injury.
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Very low
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Must monitor the air pump and notify the teacher if there is any strange sounds coming from the air pump.
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The conical flask may break
| low | Ensure the conical flasks are placed in a secure location |
5. References
https://www.thesprucepets.com/growing-out-brine-shrimp-2924614https://www.petcha.com/hatching-brine-shrimp/ http://www.yourfishstuff.com/hatching-brine-shrimp-eggs
6. Bibliography: List at least three (3) major sources (e.g. science journal articles, books, internet sites) from your literature review. Choose the APA format and use it consistently to reference the literature used in the research plan. List your entries in alphabetical order for each type of source.
(Approximately 11/2 tablespoons of salt per quart (or liter) of water. This equates to about 1.018 specific gravity as measured with a hydrometer. Aquarium, sea or non-iodized table salt can be used.) From http://www.yourfishstuff.com/hatching-brine-shrimp-eggs.
- Cut the first bottle about 2 to 3 inches from the bottom and discard the top. The bottom will become the base. (See A below.)
- Cut the bottom off the second bottle, leaving just a bit of the bottom to create a lip around what will become the top. (See B below.)
- For the third bottle, cut the bottom off about an inch from the base. This will become the cap. (See C below.) From www.petcha.com/hatching-brine-shrimp/
(Use an air pump which is powerful enough to deliver a good air supply to the bottom of the grow-out container. You will want to keep your Brine Shrimp and their food in constant motion.) From (Hauter and Stan.D)(Updated 2019)(Growing out brine shrimp for fish food) From https://www.thesprucepets.com/growing-out-brine-shrimp-2924614https://
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