Methods

2. RESEARCH QUESTION(S):

2.1 Research question being addressed

To find out what the salt concentration in the water is optimal for brine shrimp hatched.

2.2 Hypotheses

The hypothesis is the higher the salt concentration, the higher the number of shrimp eggs hatched.

2.2.1 Independent variable

The independent variable is the amount of salt added into the water.

2.2.2 Dependent variable

The number of empty eggs floating on the surface.

2.2.3 Controlled variables

(a)  The amount of water.

(b)  The number of eggs.

2.3 How is this based on the rationale described above?

The amount of salt added in the water will affect the number of eggs hatched, and this will be used to find out the optimal number of salt that needs to be inserted into the water.

3.    Method

3.1 Equipment list:

• • Salt
    
    
  • Six conical flasks
  • Air pump
    
    
  • Dissecting Microscope
    
    
  • Teaspoon

3.2 Diagrams

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Risk	Assessment	Management
The student may accidentally get the salt water into his eyes	Medium	Wear protective eyepieces.
The air pump may malfunction and cause injury.	Very low	Must monitor the air pump and notify the teacher if there is any strange sounds coming from the air pump.
The conical flask may break	low	Ensure the conical flasks are placed in a secure location

3.3 Procedures:

1. Add 250 ml of water
2. Connect the air pump to the tubing and put the tubing in the 6 beakers.
3. Cut the tops of the water bottles and put them upside down in the beaker
4. Turn on the air pump and leave the eggs to settle